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Beneficial effects of Spirulina platensis , voluntary exercise and environmental enrichment against adolescent stress induced deficits in cognitive functions, hippocampal BDNF and morphological remolding in adult female rats

*Correspondence: Yangchun Xie, [email protected] Tang, [email protected] This article was submitted to Signaling, a section of the journal Frontiers in Cell and Developmental Biology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

doi: 10.1016/j.brainresbull.2022.03.015 217 WangCHanDFengXWuJ

CRISPR sgRNA sequences targeting FSP1 were designed using the CRISPR guide design tool by Benchling ( sgRNAs targeting RFK , FLAD1 , NFE2L1 , NFE2L2 , UBR4 , KCMF1 , RNF4 , CUL1 , RNF113A , ARIH1 , RNF8 and SAFE-control were selected on the basis of the effective score from the human CRISPRCas9 KO libraries used for genetic screening