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For instance, in healthcare workers, NSW was associated with elevated low-grade systemic inflammation, evidenced by higher leukocytes, TNF-, IL-10, and LBP levels

Finally, the membranes were washed with PBST three times, and a ChemiDoc XRS System (Bio-Rad, CA, USA) was used to detect the expression of proteins with an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China)

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The endoplasmic reticulum (ER) is responsible for lipid biosynthesis in cells, arising as the main source of essential components of membrane bilayers such as phospholipids and cholesterol, which are distributed to different intracellular compartments, including mitochondria