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Description

Samples were then alkylated with 60 M IAM-TPP or IAM for 20 min at RT in the dark and then reduced with 120 M TCEP for 3 min and additionally alkylated with 240 M IAM or IAM-TPP for 20 min at RT in the dark

10.1016/j.bbi.2023.02.012 148 ZhuY.WangB.TaoK.YangH.WangY.ZhouT.et al (2017)

PMCID: PMC2803035

But then thyroid tissue regeneration is a whole other set of protocols and its not something I would say I could guarantee for everybody, and its not even a realistic goal for some people