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This FGF8 signaling cascade subsequently activates Wnt1 expression in the midbrain, which in turn upregulates the Engrailed-1/2 transcription factorsfirst detected at embryonic days 11.514 in micewhereupon their sustained expression becomes essential for the maintenance and survival of VTA DA neurons (Hegarty et al., 2013)

The combined acupoint treatment exhibited significantly superior results compared to those with individual acupoint stimulation, aligning more closely with the clinical practice of acupoint prescription

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