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It does not cross the blood-brain barrier effectively, does not specifically address cardiac muscle function, and does not carry the additional acetyl group that supports acetylcholine synthesis

As expected, the injection of antisense oligonucleotide to Ldlr -null mice decreases the hepatic Fmo3 gene expression, resulting in decreased mouse plasma TMAO thereby decreasing aortic lesion in western diet fed mice (Shih et al., 2015)