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Organoids, 2D hepatocyte monolayers or 2D sandwich cultures were then all cultured in Williams E medium supplemented with 1% HEPES + 1% GlutaMAX + 1% penicillin/streptomycin supplemented with luciferin-H substrate (100 M) or luciferin-IPA (3 M) for 6 h

Additional evidence indicates that FMT effectively mitigates microbial dysbiosis and reduces circulating uremic toxins, including p-cresyl glucuronide and PCS, which are strongly associated with CKD progression [210]

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