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During elution in this method, ionic strength increases, and pH changes occur

Data extraction was carried out using a data extraction form which was designed using the Joanna Briggs Institute (JBI) guidance on data extraction (Tufanaru et al ., Reference Tufanaru, Munn, Aromataris, Campbell, Hopp, Aromataris and Munnz2020) and the Cochrane Handbook for Systematic Reviews of Interventions (Li et al ., Reference Li, Higgins, Deeks, Higgins, Thomas, Chandler, Cumpston, Li, Page and Welch2019)

Nevertheless, there are still some disadvantages of these techniques, for example, non-specific cross-hybridization usually cause a high background level which limits the detection range, the transcripts can be detected only with high copy number, and the total coverage of the transcripts are almost unknown (Liu et al., 2015)

A study by Tverdal et al (2020) concurs that moderate intake of filtered coffee is also correlated with a lower all-cause mortality rate, when compared to regular consumption of either unfiltered brew or no coffee consumption