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Description

Applied and Environmental Microbiology, 69(9), 57225725

10.1007/s00280-003-0706-9 21 LebwohlD.CanettaR

It has been suggested that hyperbilirubinemia may have another etiology, possibly related to impaired liver clearance of bilirubin

The new supernatant (cytosolic fraction) was directly prepared for SDS-PAGE, and the pellet (membrane fraction) was gently washed with hypo-osmotic buffer and resuspended in GST-lysis buffer supplemented with 0.5% SDS and protease inhibitors