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This methodology and analysis are to be documented in a report to be filed on July 1, 2019

Astaxanthin: Sources, Extraction, Stability, Biological Activities and Its Commercial Applications 25

Gel pieces were transferred to microcentrifuge tubes and destained with 100 l of 100 mM ammonium bicarbonate/acetonitrile (1:1, vol/vol) and incubated 30 min at room temperature with occasional vortexing

In type 1 diabetes, cytokine-induced ER stress may account for impaired folding of insulin and altered HLA presentation of antigenic peptides thereof, hence contributing to beta cell dysfunction and T cell-mediated destruction [67,68,69]