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A healthy and balanced diet, regular physical activity, and other lifestyle modifications are essential for achieving sustainable weight loss

Thirty or forty micrograms of proteins were loaded into each well and fractionated on 510% precast SDS-PAGE gels (Byeotime, Shanghai, China) and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany)

Second, as all analyses were exploratory, and the primary analysis for each endpoint was specified, multiple comparisons were performed only without P value correction

For instance, different meningococcal strains differ in their capability to evoke inflammation and exploit different routes to cross the NEB and BBB