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Collectively, these mitochondrial dysfunction-associated events contribute to ferroptosis-related nucleus pulposus cell injury and extracellular matrix degradation in IDD, thereby suggesting that targeting mitochondrial dysfunction may represent a potential therapeutic strategy for IDD

Immunohistochemistry of Glutathione Reductase and Superoxide Dismutase 2 in the Kidney For immunohistochemical detection of glutathione reductase (GR) and superoxide dismutase 2 (SOD2) in the kidney, we used polyclonal anti-glutathione reductase and anti-superoxide dismutase 2, respectively (Chongqing Biospes Co., Ltd., China) and Power-Stain 1.0 Poly horseradish peroxidase (HRP) DAB Kit (Genemed Biotechnologies, Inc., 458 Carlton Ct