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In other words, the coexistence of necrotic and apoptotic markers inside the same cell shape a heterogeneous phenotype characterized by a mixture of suicide program activation 33

To gain insights into molecular changes specific to different cell types and to functionally test immune responses in young versus old killifish, as well as male and female killifish, we used cytometry, transcriptomics, proteomics and a functional in vitro assay in immune cells stimulated with endotoxin (lipopolysaccharide (LPS))

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