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For transient transfection paradigms, 4 to 15 g of cDNA coding for N -terminally Flag-tagged WT human parkin or empty Flag control vector (pcDNA3.1) using a 1:1 ratio of cDNA:Lipofectamine 2000, was used for ectopic expression

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Finally, patients who already have intestinal barrier damage need to receive specific medical treatment and to promote recovery and prevent further complications under the close supervision of a medical professional