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Cell 6 , 661672 (2004)

GST pull-down vs immunoprecipitation Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads GST pull-downs are assays used to investigate protein-protein interactions

A decrease in Steap1 expression may not favor Cu2+ reduction for subsequent transmembrane transport from endocytic organelles to the cytoplasm

Choline intake and status in patients on their habitual diet were unfortunately not assessed in the Manchester studies, but are needed urgently