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d , Immunoblot analysis of SLC33A1 using SLC33A1 antibody (generated in this paper) in whole cell and ER samples from HEK293T cells or SLC33A1 -knockout HEK293T cells

Vitamin K1 (phylloquinone) is abundant in leafy greens and is primarily used by the liver for blood clotting

Similar to previous protocol, we carried out tandem affinity purification and analyzed the protein complexes using negative stain electron microscopy, which exhibits a comparable dimeric architecture (Supplementary Fig