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Microarray analysis For each growth condition, microarray experiments were performed in two replicates with RNA isolated from independent cultures grown on different days

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But theres another catch, because cysteine cannot be given as such, we had to supplement it in another form called N-acetylcysteine. In past studies, Sekhar and his colleagues determined that supplementing GlyNAC, a combination of glycine and N-acetylcysteine, corrected glutathione deficiency inside the cells of naturally aged mice to the levels found in younger mice

Arch Toxicol 82(5):273299 Mats JM, Segura JA, Alonso FJ, Mrquez J (2012a) Oxidative stress in apoptosis and cancer: an update