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Preparation of cytoplasmic lysates for ChemRAP For each pulldown, 2 10 7 HeLa-S3-FlpIn cells were centrifuged for 5 min at 200 g at 4 C, washed twice with 25 mL of ice-cold PBS and resuspended in 500 L of CPE buffer (10 mM HEPES, pH 7.9, 10 mM KCl, 1.5 mM MgCl 2 , 0.34 M sucrose, 10% glycerol, 1 mM DTT, 0.1% Triton X-100, supplemented with EDTA-free Complete Protease Inhibitor cocktail from Roche)

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[5] She and her daughter received minor injuries

[14] Peter Anderson and his colleagues performed longitudinal studies and concluded that "alcohol advertising and promotion increases the likelihood that adolescents will start to use alcohol, and to drink more if they are already using alcohol"