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For intracellular staining, the cells were subsequently harvested, washed with PBS, fixed, and permeabilized with a FOXP3/transcription factor fixation/permeabilization kit (Thermo Fisher Scientific)

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We included PRAMS data from a large population-based sample of 16,022 women residing in 38 US sites who had a recent live birth and smoked combustible cigarettes prior to becoming pregnant