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Cells were symmetrically bathed in a modified Ringers solution (120 mM NaCl, 10 mM d-Glucose, 3.3 mM KH 2 PO 4 , 0.83 mM K 2 HPO 4 , 1.2 mM MgCl 2 , 1.2 mM CaCl 2 , 25 mM NaHCO 3 , pH 7.4)

Also, variations in color may occur due to digital processing