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Actinidine dissolved in diethyl ether was stored in the dark but at fluctuating temperatures, ranging from freezing to room temperature and was used once to test 900 g after 2 weeks of storage

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Under-reporting of substance use may have occurred due to cultural differences within regions, as women would be less likely to report substance use due to potential stigmatisation, and because the CAGE questionnaire asks sensitive questions which participants may have found difficult to answer honestly and objectively