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Results Analyzed cystic mice included Pkd1 flox/flox : Nestin cre and Pkd1 flox/- : Nestin cre animals After the establishment of our experimental groups, a surveillance protocol of genotype control identified that Nestin cre -mediated Pkd1 inactivation had gone germline in the colony, leading to the generation of some noncystic Pkd1 flox/- and some cystic Pkd1 flox/- : Nestin cre mice