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In the 1960s consumers became more aware that smoking leads to cancer, and in 1965 legislation passed that mandated that cigarette packs and advertisements have warnings

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The authors write: The observed detrimental effects in the lung upon [e-cigarette] vapour exposure in animal models highlight the need for further investigation of safety and toxicity of these rapidly expanding devices worldwide. For the tests, they exposed groups of mice to either cigarette smoke or vapours containing propylene glycol a common base ingredient in e-liquids with added vegetable glycerol, nicotine, or nicotine and tobacco flavouring