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Prophet (Peace be upon him) said: every intoxicant is khamr, and every khamr is forbidden

Preparation of cytoplasmic lysates for ChemRAP For each pulldown, 2 10 7 HeLa-S3-FlpIn cells were centrifuged for 5 min at 200 g at 4 C, washed twice with 25 mL of ice-cold PBS and resuspended in 500 L of CPE buffer (10 mM HEPES, pH 7.9, 10 mM KCl, 1.5 mM MgCl 2 , 0.34 M sucrose, 10% glycerol, 1 mM DTT, 0.1% Triton X-100, supplemented with EDTA-free Complete Protease Inhibitor cocktail from Roche)

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