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Pueblo is a premium tobacco brand produced in Germany, renowned for its additive-free products, much like American Spirit

Furthermore, it is important to realize that cigarette smoke comprises a high number of individual compounds [34], including acetaldehyde, benzene, 1,3-butadiene, and isoprene with an elevated mutagenic potential [35]

Among the 20 cigarettes per PM class and product, outliers were identified with the Iglewicz and Hoaglin's robust test for multiple outliers (one outlier for Natural American Spirit orange could be detected and then excluded from the results)

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