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Oocyte in vitro maturation GV oocytes were received from consenting patients, randomly split into three groups, and cultured using standard clinical IVM media (SAGE One-step (CooperSurgical, Canada) + 7.5 IU/mL of Menopur (Ferring, Canada)): Ctrl ( n = 96, only IVM media), THC1 ( n = 95, treated with a physiological concentration of cannabis based on previous measurements of cannabis in FF 23 : 25 ng/mL 9-THC (Sigma-Aldrich, Canada), 5 ng/mL 11-OH-THC (Sigma-Aldrich, Canada), 50 ng/mL 11-COOH-THC (Sigma-Aldrich, Canada)) and THC2 ( n = 94, treated with THC at a concentration based on previous animal studies 25,27,29 : 100 ng/mL 9-THC, 50 ng/mL 11-OH-THC, 200 ng/mL 11-COOH-THC)
