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E-cigarettes (Vaping): While not completely risk-free, e-cigarettes deliver nicotine through a vaporized liquid (e-liquid) rather than burning tobacco, reducing tar exposure

Libraries were sequenced on a HiSeq 4000 device (Illumina, San Diego, CA) using a HiSeq 3000/4000 PE Cluster Kit and two 50-cycle SBS kits (Illumina, San Diego, CA), reaching an average mean depth of 61,000 (16% CV) and 50,000 (5% CV) mapped reads per cell per sample for the acute and sub-chronic models, respectively