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Anti-malarial medication is usually started a few days before travel (so that the drug has time to reach sufficient levels in your blood), is taken at regular intervals (usually daily or weekly) for the duration of the trip and is then continued for as long as four weeks after leaving the affected region, since parasites can still emerge from the liver during this period

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After protease digestion and centrifugation (1200 rpm, 5 min), PBECs were cultured in bronchial epithelial cell medium (#3211, ScienCell, San Diego, California, USA), supplemented with 100 U/ml penicillin, 100 g/ml streptomycin and 1 bronchial epithelial cell growth supplement (#3262, ScienCell, San Diego, California, USA) using poly-L-lysine-coated flasks and employed for experiments at passage 24 without mycoplasma contamination (Additional file 2: Fig