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Isolation of RNA and Real-Time Polymerase Chain Reaction (q-PCR) RNA was isolated from freshly isolated AT2 cells, which were cultured for 810 days to transdifferentiate into the AT1-like phenotype and NCI-H441 cells grown in six-well plates (Greiner Bio-One GmbH, Frickenhausen, Germany) using Tri-Reagent (Sigma-Aldrich) according to the manufacturers instructions and as described in a previously published protocol ( ACTB (forward GACGACATGGAGAAAATCTG

"The Ghost of Cigarette Advertising Past"