tobacco plant growing conditions | Cultivation, Curing & Grading How to Grow Tobacco -

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Table 3 The crystallization solutions of ammonium sulfate Sample Protein concentration and buffer a Crystallization buffer b Temperature (K) WT-TMV-CP 32 (cleaved GST) 814 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.100.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.58.5 277/295 WT-His-TMV-CP 12 814 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.100.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.58.5 277/295 TR-His-TMV-CP 19 814 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.100.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.58.5 277/295 TR-His-TMV-CP 68 814 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.100.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.58.5 277/295 TR-His-TMV-CP 62 814 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.100.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.58.5 277/295 a The buffers were provided with filter membrane