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Human primary blood-derived monocytes (pBDMCs) were isolated from donor buffy coats using the EasySep Direct Human Monocyte Isolation Kit (Stemcell, #19669), which isolates primary CD14 + monocytes via negative immunomagnetic selection according to the manufacturers protocol (Supplementary Fig

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Diana DeGette (D-CO) and Chris Stewart (R-UT) for introducing this bipartisan legislation to help prevent young people from starting down a path that often leads to addiction, disease and premature death