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After incubation, the coverslip containing the cells were washed with PBS, fixed in Paraformaldehyde 4% for 15 min RT, then washed in PBS and permeabilized for 5 min with Buffer (0.05% Saponin in PBS- plus 3% BSA) and then incubated with blocking Solution (BSA 0,5% in PBS) 1 h RT

The ICIJ investigation has independently identified five factories, located in northwestern Russia, Ukraine, and in the Krasnodar region of southern Russia, where Kazakov was born

In 1984 Douglas produced the hit of the Christmas season, Starman, which earned lead actor Jeff Bridges an Oscar nomination