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According to a study by Everitt and Wolf titled Psychomotor Stimulant Addiction: A Neural Systems Perspective, published in the Journal of Neuroscience (2002), long-term stimulant misuse leads to dopamine system dysregulation, increasing susceptibility to mental health disorders and cognitive impairments

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The coverslips were flipped over onto ProLong diamond antifade mountant with DAPI (Invitrogen, CA, USA)-loaded slide glasses and incubated overnight at 4 C