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As the wife of Steve Martins character in the latter movie, she found an inflection for the word Georgehalf amused, half disappointed, drawing on a heroic but dwindling stock of patiencethat now feels like the force that held the American family together for an entire generation: Outside in the autumn air, though, I wasnt thinking about movies much

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For one thing, they do not distinguish fat from muscle, water, or bone

Western blot analysis Total protein was extracted with RIPA buffer, and 20 g of extracted total proteins were separated on SDS-PAGE gel and transferred onto a PVDF membrane (Millipore, Billerica, MA)