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Isolation and purification of antagonistic bacteria Isolation of antagonistic endophytes was carried out by reference to the method of Hashem et al

Approximately 1 g DNPH hydrate was weighed and added to 21.4 mL EtOH and warmed with magnetic stirring agitation

PAY ATTENTION to the angle at which youre heating as this could also lead to a broken nectar collector if your chamber is being hit with the heat at the same time