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In order to prepare scRNA-seq libraries via the Chromium system, 8,700 cells per sample were loaded onto the Chromium Controller (10x Genomics, Pleasanton, CA, USA) to capture 5,000 cells

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Particles larger or smaller than 0.3 microns are captured with even higher efficiency through different physical mechanisms

The following sociodemographic data were also obtained from the World Bank: male and female population as percent of total population, male population aged 15-29 as percent of total male population, female population aged 1529 years as percent of total female population, urban population as percent of total population, total unemployment as percent of total labor force