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That Ushaped curve looks like this: Early phase (false boost): Nicotine briefly acts like a stimulant and can inhibit aromatase the enzyme that converts testosterone into estrogen so light or early smoking can push total testosterone about 515% higher than in nonsmokers. Middle phase (numbers look fine): As the habit settles in, SHBG starts to climb, binding more testosterone, so your lab printout may still show normal or even high total T while your usable, free testosterone quietly slips. 487 ng/ml (inflection point): Recent studies (2024) have identified a specific threshold of nicotine exposure 487 ng/ml of serum cotinine

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