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In Canada, 94% of recreational cannabis users in 2017 reported consuming the drug by smoking marijuana 9 , a preparation of dried cannabis flowers and leaves 1

Briefly, RBL cells were lysed on ice using hypotonic cell lysis buffer [50 mM KCL, 0.5% Nonidet P-40 (NP-40), 25mM HEPES (ph 7.8), 1 mM phenylmethylsulfonyl fluoride, 10 g/ml leupeptin, 20 g/ml aprotinin, 1 mM DTT] at 4C for 10 min