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Libraries were sequenced on a HiSeq 4000 device (Illumina, San Diego, CA) using a HiSeq 3000/4000 PE Cluster Kit and two 50-cycle SBS kits (Illumina, San Diego, CA), reaching an average mean depth of 61,000 (16% CV) and 50,000 (5% CV) mapped reads per cell per sample for the acute and sub-chronic models, respectively

Also Read:- Harmful Effects of Smoking on Skin and Ways to Prevent It Natural Remedies for Lung Cleansing In addition to lifestyle changes, certain natural remedies can aid in cleansing your lungs post-smoking and help clean lungs after smoking naturally

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