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For systemic or gut-related protocols, abdominal subcutaneous injection is the standard approach

Since this involves tremendous cost for healthcare systems and patients, alternative measures are being explored, namely, pharmaceutical Induction of -globin, allogenic transplantation, and gene therapy

To ensure specificity and accuracy of the amplification process, both positive and negative controls were included: positive controls (PC) consisted of RNA from PCa cell lines (LNCaP, PC3 and VCaP) known to express target genes such as AR and negative controls included RNA from healthy donor PBMCs that do not express tumor-specific genes, along with no-reverse transcription (no RT) and water controls

Martens, H