Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing and TRDs are shown

and TRDs are shown

DriverMap™ AIR RNA Full-length TCR-BCR Profiling Reagent Kit (Human)

Video: Immunophenotyping of T- and B-Cell Receptors

triplicates)

Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing and TRDs are shownPooled library of ~105,000 dual sgRNA constructs targeting ~19,000 human protein coding genes. Each construct expresses two sgRNA targeting the same gene. Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five CRISPRi dual sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual: