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A no-template control reaction was included for each gene examined

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ER tracking assay HESC and 12Z cell lines were incubated with the compounds and labeled with and ER-Tracker Green (#E34251, Invitrogen, USA) (1 M) ( n = 3) in the dark for 30 min and analyzed by flow cytometry using a Becton Dickinson (San Jose, CA, USA) flow cytometer and a Beckman Coulter Cytoflex Cytometer Software (v 1.2.5.3891)

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