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Based on the amount of GSH in the lysate, we calculated the GSH concentration assuming an averaged HeLa/HT1080 cell volume of 4,000 m 3 (refs 15, 51, 52)

10.1017/S1041610211000810 71

Once activated, NLRP3 facilitates caspase-1 activation, which results in the conversion of pro-IL-1 and pro-IL-18 into their active forms, IL-1 and IL-18 [36]