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Indeed, massive apoptosis was observed in ATP7B-KO cells upon Cu treatment (see Supplementary Figs

Protein isolation and MS spectral-data acquisition Mixed BAL cells: BAL cells were resuspended in hypotonic lysis buffer with HALT protease inhibitor cocktail (Thermo Fisher Scientific), and lysed using sequential cell disruption techniques including a freezethaw at 98 C with vortexing and sonication (Sonics) on ice before buffering with 1 M triethylammonium bicarbonate (Sigma)

The intensity of MitoTracker fluorescence in Fabry kidney organoids increased after GSH treatment (Fig

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