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Louis, MO, USA), tetrahydrofuran (C 4 H 8 O, 99.9%,Cornaredo MI, Italy), donepezil hydrochloride (C 24 H 30 ClNO 3 , 98% (HPLC), Sigma Aldrich, Lu Wan Qu, Shanghai, China), streptozotocin (C 8 H 15 N 3 O 7 , 98% (HPLC), 265.22 g/mol, Sigma Aldrich, Saint Louis, MO, USA), citrate buffer (0.1 M, pH 4.5, Biodiagnostic, Egypt), O-acetylcholine iodide (C 7 H 16 INO 2 , 99%, 273.12 g/mol, BDH chemicals, Poole, Dorset, England), 5,5-Dithio-bis(2-nitrobenzoic acid) (C 14 H 8 N 2 O 8 S 2 , 98%, 396.36 g/mol, Corsham, England), ELISA kit for brain derived neurotrophic factor (BDNF) (Elabscience, Wuhan, Hubei, China), Commercial kits from biodiagnostic, Egypt were used for the measurement of MDA, GSH and SOD

(Wilmington, MA, USA), maintained and bred at the animal house of the Fondazione IRCCS Istituto Neurologico Carlo Besta in compliance with institutional guidelines

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In fact, its the leading cause of blindness in the United States