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J Thorac Cardiovasc Surg 123(1):175-83, 2002

A suspension of zoospores (#87) was collected from actively growing flasks, filtered through a 10 M syringe filter (Millipore), centrifuged, then added to 96 well plates at 5 10 4 zoospores in TGhL per well with 10 M 2-AAPA, or 0.05% DMSO control

In C2C12 myotubes, NNMT inhibitor treatment produces concentration-dependent increases in NADH levels (50% higher at 30 M) and reduces NAD+/NADH ratios by 25-40%