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Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution (0.2% oil red O in 99% isopropanol) for 30 min

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Take an extra 15 minutes to relax prior to your treatment if you have just exercised, rushed to get to your appointment, or took the stairs up to the clinic as this will affect blood pressure readings