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Galley et al., 2017)

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To generate IgG for passive transfer experiments, immunizations were performed in groups of 25 C57BL/6 mice (12 or 13 male and female, varied per group) between 7 and 10 weeks of age

Opioids have the ability to alter the development, differentiation, and function of immune cells (11, 12), and therefore blocking their activities via LDN affects the immune system