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These and other findings (64) led to the recommendation in 1992 by the United States Centers for Disease Control and Prevention that women of childbearing age consume 0.4 mg/day of folic acid to prevent NTDs (65), and the subsequent mandate in 1996 by the United States Food and Drug Administration that all cereal grain products be fortified with folic acid (66)

A previous study identified genes necessary for MAP survival in dairy calves and permitted us to select a gene to knockout that is essential for MAP survival in vivo (MAP1643, IcL ) or one which is required for intracellular growth (MAP1531c, BacA ) ( Mouse and calf infection trials were employed to evaluate the immunogenicity of two vaccine candidates, confirm the attenuation in calf, and assess the species-specific essentiality of candidate genes

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While promising, these studies are small and short-term