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Ninety-six-well MaxiSorp Immuno ELISA plates (ThermoFisher, Waltham, MA, USA) were coated with 200 ng/well hACE-2-Fc (CIM, Cuba) in 0.1 M carbonate-bicarbonate buffer (pH 9.6) and incubated overnight at 4C

It is essential for red blood cell formation and DNA synthesis

Nevertheless, the reason for why other Plasmodium species lacking asparagine-rich proteins have retained GS and whether Pf GS can be targeted for the mosquito and liver stages require further studies