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The multifunctional deacetylase SIRT1 also plays a role in protecting against DR (159)

For NAD + and NADH assays, 20 L (for NAD + assay) or 30 L (for NADH assay) of the neutralized sample extract was combined with 90 L of assay mix consisting of 50 L of 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) with 2 mM EDTA (pH 7.5), 20 L of 1.2 mM 2,6-dichlorophenolindophenol, 10 L of 20 mM phenazine methosulfate, and 10 L of 2,500 units mL 1 aldolase dehydrogenase

Liu E, Marin D, Banerjee P, Macapinlac HA, Thompson P, Basar R, et al

For the reduction of SSG-modified proteins, 480 g of the alkylated samples were prepared at a final concentration of 1 g/L in 25 mM HEPES containing 1 M urea (pH 7.5) followed by the addition of 2.5 g/mL Grx1M (C14S mutant from Escherichia coli , Cayman, No